Effects of Clove Extract as an Anesthetic on Sperm Motility Traits and Some Hematological Parameters in Prussian Carp Carassius Gibelio

To evaluate the effects of anesthesia with different concentrations of clove extract (0, 50, 200, 400 and 600 mg) on sperm function and some hematological parameters in Prussian carp, Carassius gibelio (Bloch, 1782), 30 mature male were examined. In this purpose, sperm motility traits and some hematological parameters ( Red blood cell count (RBC), hemoglobin concentration (Hb), hematocrit (PCV), white blood cell count (WBC) and the differential leukocyte count (leukogram)) were tested. The percentage of motile sperm was unaffected by anesthetic treatments, averages ranging from 71.65% to 85.67%. However, duration of motility decreased as anesthetic concentration increased, averages ranging from 34.66 to 54.33 s. There were no significant differences between values of Hb, PCV and leukogram in each treatment (p>0.05); however, WBC was significantly lower at 400 and 600 ppm clove extract treatment group (p<0.05). Moreover, RBC in these group was increased significantly (p<0.05), and at 600 ppm clove extract treatment group was higher than those of other groups (p<0.05). The results of this study show that use of clove extract in lower dosages than 400 ppm don’t affect on sperm motility traits and hematological parameters in Prussian carp.